Global & Disaster Medicine

Archive for the ‘Hazardous Materials (HAZMAT)’ Category

IM Sodium Nitrite and Sodium Thiosulfate Are Effective Against Acute Cyanide Poisoning

Sodium Nitrite and Sodium Thiosulfate Are Effective Against Acute Cyanide Poisoning When Administered by Intramuscular Injection

Vikhyat S. Bebarta, Matthew Brittain, Adriano Chan, Norma Garrett, David Yoon, Tanya Burney, David Mukai, Michael Babin, Renate B. Pilz, Sari B. Mahon, Matthew Brenner, Gerry R. Boss
DOI: http://dx.doi.org/10.1016/j.annemergmed.2016.09.034
p718–725.e4
“…..Results

We found that sodium nitrite and sodium thiosulfate individually rescued 100% of the mice, and that the combination of the 2 drugs rescued 73% of the rabbits and 80% of the pigs. In all 3 species, survival in treated animals was significantly better than in control animals (log rank test, P<.05). In the pigs, the drugs attenuated an increase in the plasma lactate concentration within 5 minutes postantidote injection (difference: plasma lactate, saline solution–treated versus nitrite- or thiosulfate-treated 1.76 [95% confidence interval 1.25 to 2.27]).

Conclusion

We conclude that sodium nitrite and sodium thiosulfate administered by intramuscular injection are effective against severe cyanide poisoning in 3 clinically relevant animal models of out-of-hospital emergency care…..”


The microgravity conditions of the International Space Station (ISS) may hold the key to improving our understanding of how to combat sarin and VX.

NIH

Monday, July 17, 2017

Space station project seeks to crystalize the means to counteract nerve poisons

NIH-supported experiment could lead to improved antidotes.

The microgravity conditions of the International Space Station (ISS) may hold the key to improving our understanding of how to combat toxic nerve agents such as sarin and VX. That is the hope of Countermeasures Against Chemical Threats (CounterACT) project that is part of an initiative at the National Institutes of Health aimed at developing improved antidotes for chemical agents.

“With increasing worldwide concern about the use of chemical weapons, there is significant interest in developing better counteragents,” said David A. Jett, Ph.D., director of the CounterACT program, National Institute of Neurological Disorders and Stroke (NINDS), a part of NIH.

Organophosphates (OPs), a family of chemicals that includes several pesticides as well as sarin and VX nerve agents, block the activity of the enzyme acetylcholinesterase (AChE). This enzyme is critical for allowing muscles to relax after they have been stimulated by the nervous system. When the activity of AChE is blocked (for example, by OPs), muscles cannot relax, leading to paralysis and eventually death.

Developing antidotes to this type of poisoning requires detailed knowledge about the structure of the AChE enzyme. Until now, the forces of gravity on Earth have posed a challenge to this area of research. That’s where traveling into space comes in.

In June of this year, samples of the human AChE enzyme were sent to the International Space Station U.S. Laboratory by a team of CounterACT scientists led by Andrey Kovalevsky, Ph.D., Oak Ridge National Laboratory in Oak Ridge, Tennessee, and Zoran Radić, Ph.D., University of California, San Diego. Using these samples, astronauts are currently growing large crystals of pure enzyme of a size that cannot be formed on Earth due to interference from gravity.

“By taking advantage of the microgravity conditions of the International Space Station, we hope to grow better, more uniform crystals that we are unable to grow on Earth,” said Dr. Kovalevsky.

Once the crystals are grown to a large enough size, they will be returned to Earth and analyzed by a sophisticated imaging method called neutron diffraction that can provide an atomic-level view of the enzyme.

“Using this technique, we will be able to get a closer look at how the enzyme interacts with pesticides and nerve agents and learn about how the bond between the two can be chemically reversed,” said Dr. Radić. “This method would not work on the smaller enzyme crystals that can be grown here.”

Antidotes to OP exposure reactivate AChE by directly breaking its chemical bond with the OP. However, the speed at which the countermeasures available today are able to do this is too slow to be fully effective. This project will help researchers to develop antidotes that break the AChE-OP bond more quickly and that can also be delivered orally, which is another key to dealing with large-scale exposure to nerve poisons.

“Developing better countermeasures against these sorts of nerve agents is a major thrust of our overall program,” said Dr. Jett. “This project is the kind of cutting-edge science we envisioned when we established the CounterACT program.”

This project is made possible through a partnership with NASA’s Center for the Advancement of Science in Space and is part of a larger UCSD-led CounterACT-funded effort that, in addition to Drs. Kovalevsky and Radić, also includes biophysicist Donald Blumenthal, PhD., University of Utah, Salt Lake City. Their goal is to use advanced biophysical techniques to overcome limitations in defining the atomic structure of AChE and develop more effective antidotes against OP-induced inhibition.

The CounterACT program is a trans-NIH effort that is led by the NINDS in close partnership with multiple NIH institutes including the National Institute of Allergy and Infectious Diseases, which provides oversight of the program, National Institute of Environmental Health Sciences, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Eye Institute, and other NIH Institutes and Centers.

This project is funded in part by the NIH Office of the Director through the NIH CounterACT program and managed by NINDS (NS083451).

The NINDS is the nation’s leading funder of research on the brain and nervous system. The mission of NINDS is to seek fundamental knowledge about the brain and nervous system and to use that knowledge to reduce the burden of neurological disease.

The NIH CounterACT Program supports research to understand fundamental mechanisms of toxicity caused by chemical threat agents and the application of this knowledge to develop promising therapeutics for reducing mortality and morbidity caused by these agents.

About the National Institutes of Health (NIH): NIH, the nation’s medical research agency, includes 27 Institutes and Centers and is a component of the U.S. Department of Health and Human Services. NIH is the primary federal agency conducting and supporting basic, clinical, and translational medical research, and is investigating the causes, treatments, and cures for both common and rare diseases. For more information about NIH and its programs, visit www.nih.gov.

NIH…Turning Discovery Into Health®

###

 


Organization for the Prohibition of Chemical Weapons (OPCW): The April 4th chemical attack which killed at least 84 Syrian civilians and left scores more foaming at the mouth involved sarin nerve agent.

LA Times


Cisplatin Analogs Confer Protection against Cyanide Poisoning

San Diego Union Tribune

Cisplatin Study

“….The binding affinity of the cyanide anion for the positively charged metal platinum is known to create an extremely stable complex in vitro. We therefore screened a panel of diverse cisplatin analogs and identified compounds that conferred protection from cyanide poisoning in zebrafish, mice, and rabbits. Cumulatively, this discovery pipeline begins to establish the characteristics of platinum ligands that influence their solubility, toxicity, and efficacy, and provides proof of concept that platinum-based complexes are effective antidotes for cyanide poisoning…..”


President Bashar al-Assad of Syria: Videos of victims, including dead and convulsing children, had been faked.

NY Times

 

 

 


Turkish Health Ministry: “According to the results of preliminary tests, patients were exposed to chemical material (Sarin).”

NY Times

“….The Turkish statement said the sarin conclusion had been based on autopsies on three victims performed at Turkey’s Adana Forensic Medicine Institution with the participation of representatives from the World Health Organization and the Organization for the Prohibition of Chemical Weapons, a group based in The Hague that monitors compliance with the global treaty that bans such munitions…..”

 

 


Syria: Witnesses to the attack said it began just after sunrise. Numerous photographs and graphic videos posted online by activists and residents showed children and older adults gasping and struggling to breathe, or lying motionless in the mud as rescue workers ripped off victims’ clothes and hosed them down. The bodies of least 10 children lay lined up on the ground or under a quilt.

NY TImes

“…..Rescue workers from the White Helmets civil defense organization said that many children were among at least 50 dead and 250 wounded. Radi Saad, who writes incident reports for the group, said that volunteers had reached the site not knowing a chemical was present, and that five of them had suffered from exposure to the substance…..”


A different chemical attack in Syria? This time, people collapsed outdoors, and in much larger numbers. The symptoms were also different: They included the pinpoint pupils of victims that characterize nerve agents and other banned toxins. One doctor posted a video of a patient’s eye, showing the pupil reduced to a dot. Several people were sickened simply by coming into contact with the victims.

NY Times

“….Numerous photographs and graphic videos posted online by activists and residents showed children and older adults gasping and struggling to breathe, or lying motionless in the mud as rescue workers ripped off victims’ clothes and hosed them down. The bodies of least 10 children lay lined up on the ground or under a quilt.

Rescue workers from the White Helmets civil defense organization said that many children were among at least 50 dead and 250 wounded…..”


Federal authorities have confirmed that a cyanide trap intended to kill coyotes in eastern Idaho instead killed a dog in an incident that local law enforcement officials say also injured a 14-year-old boy.

MSN

 

“….M-44s are spring-activated devices typically smeared with bait that shoot poison into an animal’s mouth when it tugs on the trap. The federal government uses the devices to control predator populations in an effort to limit losses to livestock operators….”


March 20, 1995: In Tokyo, 12 people were killed and more than 5,500 others sickened when packages containing sarin leaked on 5 separate subway trains.


Categories

Recent Posts

Archives

Admin